cells hydrocyclone cell diameter hydrocyclones Prior art date Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.) Expired - Fee Related, expires Application number US10/275,086 Other ...
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A 3D-printed mini-hydrocyclone for high throughput particle separation: application to primary harvesting of microalgae ... as well as experimentation of 3D-printed miniaturized hydrocyclones with smaller cut-size for high-throughput particle/cell sorting. The characteristics of the mini-cyclones were numerically investigated using ...
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Here, we demonstrate the design, fabrication and evaluation based on CFD simulation as well as experimentation of 3D-printed miniaturized hydrocyclones with smaller …
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Therefore, a hydrocyclone membrane filtration cell (HMFC) enabled by 3D- technology was developed in this study and examined for its capability and mechanisms for pretreatment- and ...
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The small 10 cm – high Cell Culture Hydrocyclone has a maximum process-specific perfusion performance of 450–720 liters of cell culture harvest per day while operating at a …
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However, harvesting the microalgal cells from its culture medium is a huge bottleneck for its widespread commercialization. Harvesting of microalgae includes separation of the microalgal solid cells from the dilute algal suspension of 0.02–0.05% dry solids concentration. ... Hydrocyclone. Hydrocyclone is a cylindrical hopper-type … Details
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The cell concentration continuously increased up to 660 B. Schröder et al. Fig. 2 Model of the cell culture hydrocyclone Overflow Hydrocyclone Harvest Pump Underflow Recirculation Pump Feed P Harvest Controller Medium Tank 200-L Bioreactor Fig. 3 Illustration of the perfusion system Advantages of Hydrodynamic Cell Separation in Industrial Cell ...
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To showcase its utility, high-throughput algae harvesting from the medium with low energy input is demonstrated for the marine microalgae Tetraselmis suecica. Final microalgal biomass concentration was increased by 7.13 times in 11 minutes of operation time using our designed hydrocyclone (HC-1).
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O-grid structured hexahedral mesh for the simulated hydrocyclone membrane filtration cell. Download: Download high-res image (212KB) Download: Download full-size image; Fig. 3. Effect of mesh node number on the simulated radial distributions of tangential (a) and axial (b) flow velocity in the hydrocyclone membrane filtration cell at z = 20 mm.
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The small 10 cm – high Cell Culture Hydrocyclone has a maximum process-specific perfusion performance of 450–720 liters of cell culture harvest per day while operating at a …
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In our previous study, we proposed a 3D-printed micro-hydrocyclone that could be applied to the primary harvesting of microalgae cells. 31 In that study, however, the flow inside the micro-hydrocyclone could not be observed due to the opaque materials used in the device. However, we realized that without being able to visualize the particle ...
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The small 10 cm – high Cell Culture Hydrocyclone has a maximum process-specific perfusion performance of 450–720 liters of cell culture harvest per day while operating at a flow rate of 0.3 ...
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Due to the more cell density harvesting cost generally decreases in this system of cultivation (Iasimone et al., 2020). ... The hydrocyclone is cylindrical equipment where the input microalgae culture is fed at the top from an angle and the cells move downward through the vessel in a cyclonic motion, larger and denser particles dragging towards ...
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Cell passage through the hydrocyclone did not induce apoptosis. The high separation efficiencies coupled to the low ... As the overflow is commonly used for product harvest and cells are discarded, the application of the hydrocyclone has no detrimental effect on the reactor perfusion system. The results indicate that only cells passing from the ...
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EP4229174A1 EP21802519.5A EP21802519A EP4229174A1 EP 4229174 A1 EP4229174 A1 EP 4229174A1 EP 21802519 A EP21802519 A EP 21802519A EP 4229174 A1 EP4229174 A1 EP 4229174A1 Authority EP European Patent Office Prior art keywords bioreactor cells culture production cell Prior art date Legal status (The legal status is an assumption and is …
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Europe PMC is an archive of life sciences journal literature.
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To showcase its utility, high-throughput algae harvesting from the medium with low energy input is demonstrated for the marine microalgae Tetraselmis suecica. Final microalgal biomass concentration was increased by …
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However, harvesting the microalgal cells from its culture medium is a huge bottleneck for its widespread commercialization. Harvesting of microalgae includes separation of the microalgal solid cells from the dilute algal suspension of 0.02–0.05% dry solids concentration. ... Hydrocyclone. Hydrocyclone is a cylindrical hopper-type equipment ...
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Cell Harvesting Centrifugation. One of the most common forms of cell harvesting is centrifugation. Centrifugation is the process of spinning a solution continuously until substances separate themselves. By using the density difference between solids and fluids, the process of sedimentation is accelerated, meaning more dense particles move to ...
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Stage 1 (harvesting/primary concentration): increases the biomass concentration by a concentration factor of 10–20. The material retains its fluid like consistency. Stage II (thickening): thickens the primary concentrate by an additional concentration factor of 10 and generates a material with slurry-like consistency.. Stage III (dewatering): dewaters the …
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Therefore, a hydrocyclone membrane filtration cell (HMFC) enabled by 3D- technology was developed in this study and examined for its capability and mechanisms for pretreatment- and backwash-free filtration of inorganic and organic particle suspensions. ... for energy-efficient harvesting of clean water from particle-laden water ...
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Hydrocyclone has a history of about 129 years. It has been used in various industries including mineral, chemical, coal, petroleum, papermaking, environmental protection, soil remediation, waste management, agriculture, aquaculture, food, biotechnology, nanotechnology, material science technology, and thermal energy due to its high separation …
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As the overflow is commonly used for product harvest and cells are discarded, the application of the hydrocyclone has no detrimental effect on the reactor perfusion system. ... for optimum dimensions. J Petrol Sci Eng, 1994; 11: 37-50. [36] Dhamo N. Electrochemical oxidation of cyanide in the hydrocyclone cell. Waste Manag, 1996; 16: 257-61 ...
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To help obtain a high concentration ratio and achieve satisfactory removal efficiency, we review valuable measures such as non-standard unit design, geometry and …
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hydrocyclone mammalian cell retention device for and reusable bioreactors perfusion technologies overflow (harvest) feed feed product specification material. ... prior to harvest due to long residence times in. the bioreactor. Perfusion mode is an alterna-tive process operation methodology which.
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Hydrocyclone is a simple, low-cost equipment widely employed for separating aqueous particles by using a swirling flow field [14]. However, the separation cut-off of industrial hydrocyclones is generally above 3 μm [15], much larger than the size of colloidal particles (i.e., 0.001–1 μm) commonly present in water and wastewater.
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To showcase its utility, high-throughput algae harvesting from the medium with low energy input is demonstrated for the marine microalgae Tetraselmis suecica. Final microalgal biomass concentration was increased by 7.13 times in 11 minutes of operation time using our designed hydrocyclone (HC-1).
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Gregg et al. [138] reported a low harvesting efficiency for particles smaller than 400 μm using hydrocyclones for the harvesting of green algae. In terms of cell damage, a hydrocyclone is predicted to cause a certain level of mechanical disruption, however, there is a lack of specialized studies on its effect for large colonial microalgae [139].
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This work investigates different hydrocyclone configurations for separating Chinese hamster ovary (CHO) cells through an experimental design. Cell separation efficiencies remained above 97% for both wildtype CHO.K1 and recombinant CHO cell lines producing GM-CSF, with low viability losses as indicated by trypan blue exclusion and LDH release.
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